Heat-shock induction of MIF-GFP does not induce proliferation under homeostatic conditions.

<p>(A) Schema of the Tol2 construct used to drive human MIF-turboGFP downstream of the hsp70 promoter. Encoded within the genetic construct is a green heart marker using cmlc2:EGFP to initially pick select larvae with the construct. All constructs were co-injected with transposase mRNA. (B) Representative large-field images of a clutch of zebrafish larvae before and after heat-shock induction of MIF-GFP. (C) A 10× confocal image of the distribution of MIF-GFP in a larvae pre and post heat shock induction. (D) Representative images depicting MIF antibody staining with and without heat-shock inducible MIF, under undamaged (DMSO) and damage (MTZ) conditions. (E) The mean fluorescent intensity between DMSO and MTZ conditions in non-injected larvae and hsp70l:Hsa.MIF-GFP injected larvae. There are 3 ROIs selected per tail fin. n = 67, No injection, DMSO; n = 40, Hsp70:MIF-GFP, DMSO; n = 51, No injection, MTZ; n = 38, hsp70l:Hsa.MIF-GFP, MTZ. ****<0.0001, **0.0011, *0.0401 using one-way ANOVA. (F) A comparison of proliferation in heat-shocked and non-heat shocked larvae. n = 36, no inj. n = 32, heat shock. A student’s t test was used to assess differences in means. Scale bars: B = 100 μm, C = 500 μm, D = 50 μm.</p> <p>(PDF)</p>