Characterization of the regenerative response after MTZ treatment.

<p>(A) Assessment of apoptosis-induced proliferation between uninjected larvae and <i>tyrosinase</i> CRISPR larvae after the addition of MTZ. A two-way ANOVA demonstrates that there is no statistical difference between the means for MTZ-treated uninjected and <i>tyrosinase</i> FO CRISPR. <i>n</i> = 41, uninjected, DMSO. <i>n</i> = 59 uninjected, MTZ. <i>n</i> = 23 <i>tyrosinase</i> CRISPR, DMSO. <i>n</i> = 40 <i>tyrosinase</i> FO CRISPR, MTZ. (B) Quantification of esAEVs produced across all conditions by 6 hpt. <i>n</i> = 36, DMSO; <i>n</i> = 24, MTZ; <i>n</i> = 31, MTZ + 4-IPP; <i>n</i> = 22, MTZ+ISO-1; <i>n</i> = 25, MTZ + Anti-CD74; <i>n</i> = 20, <i>tyr</i> FO CRISPR; <i>n</i> = 20, <i>mif</i> FO CRISPR; <i>n</i> = 17, <i>cd74a</i> FO CRISPR; <i>n</i> = 22, <i>cd74b</i> FO CRISPR. **** <i>p</i> < 0.0001 via a one-way ANOVA using a Tukey’s ad hoc test. (C) A representative image of the regions where Tp63 cells were quantified. (D) Representative images of Tp63 positive nuclei per condition. (E) Quantifications of the number of Tp63 positive nuclei in wild-type (WT) and <i>mif</i> crispr conditions at 5 dpf. 5 dpf: <i>n</i> = 66, WT, DMSO; <i>n</i> = 44, <i>mif</i> CRISPR, DMSO; <i>n</i> = 56, WT, MTZ; <i>n</i> = 54, <i>mif</i> CRISPR, MTZ. Adjusted <i>p</i>-values: ****<0.0001 via a one-way ANOVA. (F) Percent survival of larvae before and after MTZ treatment. Scale bar = 50 μm. The underlying data for the graphs in this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002194#pbio.3002194.s017" target="_blank">S2 Data</a>.</p> <p>(PDF)</p>