Autolysis in 50 mM HEPES buffer (pH 8.5).

(A) Against <i>N</i>. <i>gonorrhoeae</i> in plain buffer. The data shown here are the mean values of 8 biological replicates, except for Δ<i>napRABC napI</i>::<i>kanR</i> (<i>n</i> = 4). Standard deviation are shown in transparent corresponding colours. One-way ANOVA was performed on data from T = 75 min, with Dunnett’s multiple comparison against the WT values (<i>p</i> < 0.033, *; <i>p</i> < 0.002, **; <i>p</i> < 0.001, ***). (B) Against <i>N</i>. <i>gonorrhoeae</i> in buffer supplemented with 10 mM MgCl₂ (<i>n</i> = 3). (C) Against <i>N</i>. <i>cinerea</i> in plain buffer (<i>n</i> = 3). (D) Against <i>N</i>. <i>cinerea</i> in buffer supplemented with 10 mM MgCl₂ (<i>n</i> = 3). The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003001#pbio.3003001.s022" target="_blank">S10 Data</a>. (PDF)