Degron-mediated depletion of RBM-26 in PLM.

<p>L4 <i>rbm-26 (syb2552); resi7</i> worms that express RBM-26::Scarlet::AID and F-box Transport Inhibitor Response 1 (TIR-1) in neurons were placed in normal NGM plates (A–C) or on plates coated with 4 mM synthetic auxin (D, E), allowed to lay eggs and L3 progenies were analyzed through confocal microscopy. PLM neuron was visualized with the help of the <i>muIs32 (Pmec-7</i>::<i>gfp)</i> transgene. Scale bars are 10 μm. Auxin treatment causes depletion of RBM-26 protein, which was quantified in G as a ratio of RBM-26::Scarlet intensity relative to the GFP expression in PLM. Underlying data can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002876#pbio.3002876.s017" target="_blank">S2 Data</a>.</p> <p>(PDF)</p>