Loss of MAP4K4 expression does not affect the number of hematopoietic stem and progenitor cells, erythrocytes, and platelets.

(A) Map4k4-deficient mice model. (B) RT-qPCR analysis of <i>Map4k4</i> knockdown efficiency in mice BM cells (n=4; mean ± SD). Unpaired Student’s t-test. (C) Bone marrow cell numbers control (Ctrl) or <i>Map4k4</i>-cKO (cKO) mice; BM, bone marrow; (Ctrl n=7, cKO n=6; mean ± SD). (D) Percentage of hematopoietic stem and progenitor cells in Bone marrow of control (Ctrl) or <i>Map4k4</i>-cKO (cKO) mice; LT-HSC, Long-term hematopoietic stem cells; ST-HSC, Short-term hematopoietic stem cells; MPP, Multipotent blood progenitors; CMP, Common Myeloid Progenitor; GMP, Granulocyte-Macrophage Progenitor; BM, bone marrow; (Ctrl n=7, cKO n=6; mean ± SD). (E) Numbers of hematopoietic stem and progenitor cells in Bone marrow of control (Ctrl) or <i>Map4k4</i>-cKO (cKO) mice; LT-HSC, Long-term hematopoietic stem cells; ST-HSC, Short-term hematopoietic stem cells; MPP, Multipotent blood progenitors; CMP, Common Myeloid Progenitor; GMP, Granulocyte-Macrophage Progenitor; BM, bone marrow; (Ctrl n=7, cKO n=6; mean ± SD). (F) The number of primitive erythroid progenitor cells (BFU-E) colonies formed by 25,000 whole bone marrow cells from control (Ctrl) or <i>Map4k4</i>-cKO (cKO) BM, bone marrow; (n=4; mean ± SD). (G) Numbers of Eos cells in the Bone marrow of control (Ctrl) or <i>Map4k4</i>-cKO (cKO) mice; BM, bone marrow; Eso, eosinophils; (Ctrl n=7, cKO n=6; mean ± SD). (H) Numbers of Mon cells in the Bone marrow of control (Ctrl) or <i>Map4k4</i>-cKO (cKO) mice; BM, bone marrow; Mon, monocyte (Ctrl n=7, cKO n=6; mean ± SD). (I) PB LYM of control (Ctrl) or <i>Map4k4</i>-cKO (cKO) mice; PB, peripheral blood; LYM, lymphocyte (Ctrl n=7, cKO n=6; mean ± SD). (J) PB RBC numbers, HGB, MCH, and MCV of control (Ctrl) or <i>Map4k4</i>-cKO (cKO) mice; PB, peripheral blood; RBC, Red blood cell; HGB, Hemoglobin; MCH, Mean corpuscular hemoglobin; MCV, Mean corpuscular volume; (Ctrl n=7, cKO n=6; mean ± SD). (K) PB PLT, PCT of control (Ctrl) or <i>Map4k4</i>-cKO (cKO) mice; PB, peripheral blood; MPLT, Platelets; PCT, Plateletcrit; (Ctrl n=7, cKO n=6; mean ± SD). (L) Numbers of DCs in the spleen of control (Ctrl) or <i>Map4k4</i>-cKO (cKO) mice; SP, Spleen; DCs, Dendritic cells; (Ctrl n=7, cKO n=6; mean ± SD). (M) Numbers of Mø in the spleen of control (Ctrl) or <i>Map4k4</i>-cKO (cKO) mice; SP, Spleen; Mø, Macrophage; (Ctrl n=7, cKO n=6; mean ± SD). Mann-Whitney U test. (PDF)