Application of enzyme-responsive fluorescent probes in detecting drug-resistant bacteria

With the overuse of clinical antibiotics, the types and varieties of drug-resistant bacteria are continually increasing. The timely and accurate identification of bacterial strains is crucial for the prevention and control of drug-resistant bacteria and the development of antibiotics. Fluorescent probes for detecting key bacterial enzyme metabolites,as a novel detection tool, can overcome the limitations of traditional detection methods and show great potential and advantages in detecting drug-resistant bacteria. This paper focuses on fluorescent probes for detecting key enzyme metabolites of clinically common drug-resistant bacteria, summarizing the applications and principles of enzyme-responsive fluorescent probe technology in detecting drug-resistant bacteria in recent years. The study reviews the relationship between bacterial resistance mechanisms and enzyme metabolites, highlighting the differences in enzyme metabolism between resistant and sensitive bacteria. Subsequently, based on the analysis of characteristic enzyme metabolites of various drug-resistant bacteria, the working principle of fluorescent probes for detecting drug-resistant bacteria is elucidated. Additionally, the study explores possible directions for optimizing fluorescent probes, providing new insights for faster and more accurate diagnosis and treatment of drug-resistant bacteria.