Use of rocker bioreactor for propagation of the

\nPlant biotechnology has the potential to meet the market demand for a significant number of seedlings. The current state of the industry is characterised by exemplary results in the field of micropropagation, with notable achievements observed in the cultivation of diverse plant crops, particularly berries. Nevertheless, certain aspects of this technology remain imperfect at the industrial level. The multiplication of microshoots of berry crops remains a costly process. The transition of numerous laboratories from semi-solid agar medium to liquid has resulted in a reduction in the costs associated with propagation. However, the majority of standard bioreactors are costly and frequently challenging to maintain. In the present study, a rocker-type bioreactor system for micropropagation of blueberries was tested using Vaccinium corymbosum L. ‘Pink Lemonade’ as an example. In comparison to the traditional method of micropropagation on a gel medium, the rocker-type bioreactor demonstrates superior outcomes. The multiplication coefficient of blueberry shoots was found to be approximately twofold higher when the liquid WPM medium was supplemented with 0.2 mg/l zeatin within the bioreactor, in comparison to the control variant cultivated on a semi-solid medium of identical composition. The shoots obtained in the bioreactor successfully completed the stages of rooting and acclimatisation to post-aseptic conditions.\n

Publication Details

Journal
Springer Link (Chiba Institute of Technology)
Published
2024-10-09
DOI
https://doi.org/10.1051/bioconf/202413001027/pdf
Primary Topic
Plant pathogens and resistance mechanisms
Type
article
Field-Weighted Citation Impact
0.00

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article

Use of rocker bioreactor for propagation of the

Springer Link (Chiba Institute of Technology)
Plant pathogens and resistance mechanisms
article

Use of rocker bioreactor for propagation of the

article en

Abstract

\nPlant biotechnology has the potential to meet the market demand for a significant number of seedlings. The current state of the industry is characterised by exemplary results in the field of micropropagation, with notable achievements observed in the cultivation of diverse plant crops, particularly berries. Nevertheless, certain aspects of this technology remain imperfect at the industrial level. The multiplication of microshoots of berry crops remains a costly process. The transition of numerous laboratories from semi-solid agar medium to liquid has resulted in a reduction in the costs associated with propagation. However, the majority of standard bioreactors are costly and frequently challenging to maintain. In the present study, a rocker-type bioreactor system for micropropagation of blueberries was tested using Vaccinium corymbosum L. ‘Pink Lemonade’ as an example. In comparison to the traditional method of micropropagation on a gel medium, the rocker-type bioreactor demonstrates superior outcomes. The multiplication coefficient of blueberry shoots was found to be approximately twofold higher when the liquid WPM medium was supplemented with 0.2 mg/l zeatin within the bioreactor, in comparison to the control variant cultivated on a semi-solid medium of identical composition. The shoots obtained in the bioreactor successfully completed the stages of rooting and acclimatisation to post-aseptic conditions.\n

Springer Link (Chiba Institute of Technology)
Ministry of Science and Higher Education of the Russian Federation
Industry, innovation and infrastructure
Openalex Percentile: Top 39%
Plant pathogens and resistance mechanisms
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Use of rocker bioreactor for propagation of the · Springer Link (Chiba Institute of Technology) (2024) | TGRS Research Map | TGRS