Optimization of Inositol Extraction from Japanese\nRice Bran and Its Potential Application in Inhibiting Calcium Oxalate\nCrystallization for Urolithiasis Prevention

Rice\nbran is considered as one of the most prominent byproducts\nof the rice milling industry. It constitutes various bioactive compounds\nlike myo-inositol. This study focuses on extracting myo-inositol from\nJapanese rice bran (Oryza sativa Japonica\nGroup) using an eco-friendly, optimized method. The extraction was\nfirst optimized through the response surface methodology, yielding\nmaximum inositol after 2 h at 80 °C with a solute concentration\nof 1:10. Microwave-assisted extraction further improved efficiency,\nachieving a significantly higher yield in just 5 min. The inositol-rich\nextract inhibited calcium oxalate crystallization, proving effective\nagainst urolithiasis by converting calcium oxalate monohydrate to\nthe more easily excretable dihydrate form. These findings suggest\nthe potential for developing cost-effective, eco-friendly formulations\nand nutraceuticals to combat urolithiasis.