Immobilization of\nCoupled Enzymes onto Metalated Hierarchical\nOrganic Microspheres

Hierarchical organic microspheres (HOMs) have emerged\nas an ideal\ncarrier for immobilizing biomacromolecules. In this research, an in-depth\ninvestigation into the structural characteristics of a striated HOM,\nknown as HOM-15, has revealed the assembly mechanism of microspheres\nthrough weakly stacked two-dimensional structural units that are composed\nof V-shaped small organic molecules. With the leverage of this understanding,\nHOM-15 was adopted as a stable and reusable platform for co-immobilizing\nof ene-reductases and glucose dehydrogenases via metal ion bridging\nonto the surface of HOMs. The research demonstrates that metal ion\nbridging can finely tune the surface properties of HOM-15, thereby\nfacilitating the immobilization of enzymes that would otherwise be\nimpeded by electrostatic repulsion. Comparing HOM-15 to other microspherical\nvariants revealed its superior biocatalytic performance, attributed\nto the reduction of the mass transfer barrier facilitated by its lamellar-stacking\nmorphology. This novel biocatalytic system underscores the potential\napplications of HOMs in broader biocatalytic processes.