Features of morfogenesis of

\nMany species of the genus Thymus are widely known as valuable essential oil and medicinal plants that have antiseptic, anti- inflammatory and analgesic properties. The use of biotechnological methods makes it possible to increase the efficiency of traditional methods of breeding and seed production. Our investigation aimed to study the influence of the different factors on the explants morphogenesis at the 2th stage of Thymus marschallianus Willd. and Thymus × citriodorus (Pers.) Schreb. clonal micropropagation. It was established that the highest morphogenetic potential was possessed by explants of T. marschallianus, whose multiplication index reached 19.9, while that of T. × citriodorus was 10.3. The optimal culture media for T. marschallianus (½MS with 0.5 mg/l naphthylacetic acid and 20 g/l glucose) and for T. × citriodorus (MS hormone free and 20 g/l sucrose) were determined. The efficiency of culturing T. × citriodorus explants in glass jars (closed with foil) was demonstrated, which allowed increasing the multiplication index up to 2.9–6.2 times compared to using test tubes or flasks as culture vessels. It was found that a longer growing cycle (70 days) in T. × citriodorus contributed to an increase the multiplication index by 3.6 times, and in T. marschallianus – by 6.2 times compared to the standard growing cycle (40 days).\n

Publication Details

Journal
Springer Link (Chiba Institute of Technology)
Published
2024-11-21
DOI
https://doi.org/10.1051/bioconf/202414101006/pdf
Primary Topic
Genetic and Environmental Crop Studies
Type
article
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article

Features of morfogenesis of

Springer Link (Chiba Institute of Technology)
Genetic and Environmental Crop Studies
article

Features of morfogenesis of

article en

Abstract

\nMany species of the genus Thymus are widely known as valuable essential oil and medicinal plants that have antiseptic, anti- inflammatory and analgesic properties. The use of biotechnological methods makes it possible to increase the efficiency of traditional methods of breeding and seed production. Our investigation aimed to study the influence of the different factors on the explants morphogenesis at the 2th stage of Thymus marschallianus Willd. and Thymus × citriodorus (Pers.) Schreb. clonal micropropagation. It was established that the highest morphogenetic potential was possessed by explants of T. marschallianus, whose multiplication index reached 19.9, while that of T. × citriodorus was 10.3. The optimal culture media for T. marschallianus (½MS with 0.5 mg/l naphthylacetic acid and 20 g/l glucose) and for T. × citriodorus (MS hormone free and 20 g/l sucrose) were determined. The efficiency of culturing T. × citriodorus explants in glass jars (closed with foil) was demonstrated, which allowed increasing the multiplication index up to 2.9–6.2 times compared to using test tubes or flasks as culture vessels. It was found that a longer growing cycle (70 days) in T. × citriodorus contributed to an increase the multiplication index by 3.6 times, and in T. marschallianus – by 6.2 times compared to the standard growing cycle (40 days).\n

Springer Link (Chiba Institute of Technology)
Openalex Percentile: Top 40%
Genetic and Environmental Crop Studies
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