Rapid Real-Time\nPCR Based on Core–Shell Tecto-Dendrimer-Entrapped\nAu Nanoparticles

Rapid real-time PCR (generally <1 h) has broad prospects.\nIn\nthis study, we synthesized a new type of nanomaterial core–shell\ntecto-dendrimer coated with Au nanoparticles (Au CSTDs) for research\nin this field. The experimental results showed that Au CSTDs could\nsignificantly shorten the time of real-time PCR (from 72 to 28 min)\nwith different templates, while the detection limit reached 10 copies\nand the nonspecific amplification was significantly reduced. Furthermore,\nexperimental analyses and theoretical studies using the finite element\nsimulation method confirmed that Au CSTDs function by synergistically\nenhancing electrostatic adsorption and thermal conductivity. These\nproperties play a key role in improving real-time PCR, especially\nin particle–particle interactions. This study contributes an\nadvanced method to rapid real-time PCR, which is expected to remarkably\nimprove the efficiency, lower the detection limit, and enhance the\nspecificity of molecular detection.