Enhanced Humification\nEfficiency and Lignocellulose\nDegradation by Cattle-Chicken Manure Co-Composting System: Microbiome\nIn-Depth Analysis

Traditional composting faces issues such as slow degradation\nof\nlignocellulose and poor humification, which are closely related to\nthe microorganisms in the compost. However, the microbial succession\nand mechanisms involved are still not well understood. This study\nestablished a cattle-chicken manure co-composting system and investigated\nthe microbial community succession process in this system through\nmicrobial community diversity analysis. The microbial community prediction\nmodel, based on phylogenetic bin analysis, network modeling, and protein\nfunction prediction, is used to infer the mechanisms of microbial\ncommunity assembly in the system. This helps explain how the system\naccelerates the degradation and conversion of lignocellulose and the\nformation of humic acids. The results indicate that during community\nsuccession, the system enhanced microbial protein functions, strengthened\ncooperation among microbial communities, and increased the stability\nof community assembly. This ultimately led to an increase in the cellulose\ndegradation rate by 12.54%, hemicellulose degradation rate by 21.12%,\nlignin degradation rate by 9.97%, and humic acid content by 36.82%.\nThis study demonstrates the value of the system and provides new insights\ninto the mixed treatment of solid organic waste in the future.