Metabologenomics-Inspired\nDiscovery and Combinatorial\nBiosynthesis-Based Diversification of Fungal <i>O</i>‑Glycosylated\nDepsides

Through metabologenomics mining, we prioritized <i>Exophiala\nxenobiotica</i> SDU 039, a deep-sea sediment-derived fungus producing <i>O</i>-glycosylated depsides (<b>1</b>–<b>9</b>), including seven new species with varying aliphatic chains. Heterologous\nexpression validated the <i>exo</i> gene cluster, and <i>in vitro</i> enzyme assays elucidated the function of glycosyltransferase\nExoC. The chemical diversity of <i>O</i>-glycosylated depsides\nis expanded by combinatorial biosynthesis using homologues depside\nbiosynthetic genes and <i>in vitro</i> transformation with\nExoC and different sugars as substrate.