Peroxynitrite-Responsive\nNear-Infrared Fluorescent\nImaging Guided Synergistic Chemo-Photodynamic Therapy via Biomimetic\nMetal–Organic Frameworks

Peroxynitrite\n(ONOO<sup>–</sup>) plays a crucial\nrole in\nmaintaining cellular redox homeostasis and regulating diffusive processes,\ncellular transport, and signal transduction. Extensive studies have\nrevealed that increased ONOO<sup>–</sup> levels during tumor\nprogression are associated with heightened levels of oxidative stress.\nHowever, current methods lack noninvasive visualization, immediate\nreporting, and highly sensitive fluorescence sensing. In light of\nthis, we have designed a biomimetic fluorescent nanoplatform, named\nZ-C-T@CM, for peroxynitrite-responsive near-infrared fluorescent imaging\nguided cancer treatment. The nanoplatform comprises tetrakis(4-carboxyphenyl)\nporphyrin (TCPP) and curcumin (CCM) encapsulated within a zeolitic\nimidazolate framework-8 (ZIF-8), which is coated with a mouse breast\ncancer cell membrane for enhanced biocompatibility and targeting,\nwhile evading immune clearance. In vitro experimental results demonstrate\nthat the as-prepared nanoplatform exhibits enhanced near-infrared\nfluorescence emission upon exposure to ONOO<sup>–</sup>, indicating\na significant potential for noninvasive in vivo imaging of ONOO<sup>–</sup> during tumor progression. Additionally, Z-C-T@CM readily\ndegrades in the tumor microenvironment, releasing TCPP and CCM, enabling\na synergistic chemo-photodynamic therapy with near-infrared illumination.\nFurther investigations indicate that Z-C-T@CM efficiently stimulates\na tumor immune response and facilitates therapeutic efficiency. Collectively,\nthis work introduces a novel noninvasive strategy for ONOO<sup>–</sup> detection, shedding new light on the integration of cancer diagnosis\nand efficient treatment.