Scavenging of Methylglyoxal\nby the Total Flavonoids\nof Apocyni Veneti Folium in Mice

Scavenging MGO has been considered as an effective strategy\nfor\npreventing atherosclerosis. A previous study showed that the total\nflavonoids of Apocyni Veneti Folium (TFAVF) had a significant antiatherosclerotic\neffect. However, there are no studies that have investigated the MGO\nscavenging capacities of TFAVF in mice. We found that TFAVF consisted\nmainly of quercetin glycosides and kaempferol glycosides using ultrahigh\nperformance liquid chromatography coupled to quadrupole time-of-flight\ntandem mass spectrometry (UHPLC-QTOF-MS/MS). TFAVF was first demonstrated\nto effectively scavenge MGO in mice based on the formation of mono-MGO-quercetin,\nmono-MGO-dehydroquercetin, mono-MGO-isorhamnetin, mono-MGO-dehydroisorhamnetin,\nmono-MGO-kaempferol, and mono-MGO-dehydrokaempferol. In addition,\none mono-MGO-quercetin was separated and purified, and its structure\nwas elucidated as 8-MGO-quercetin based on UHPLC-QTOF-MS/MS and NMR\ndata. Quantification studies have demonstrated that kaempferol, dehydrokaempferol,\nquercetin, dehydroquercetin, isorhamnetin, and dehydroisorhamnetin\ncan dose dependently scavenge MGO in mice. Taken together, these results\nindicated that TFAVF showed a significant antiatherosclerotic effect,\nwhich might be based on MGO detoxification.