Multiplexed\nQuantitative Screening of the Cellular\nUptake of Proteins Delivered by Polymeric Nanocarriers

Proteins are important therapeutic agents, yet better\nmethods are\nneeded to deliver them inside of cells. Polymeric nanocarriers (PNCs)\nare versatile materials for this purpose, and to enhance their development,\nit is necessary to quantify protein delivery efficiency into cells\nby numerous PNC designs at the same time. Current strategies for screening\nPNC systems are qualitative and mostly serial. Here, we describe a\nmultiplexed approach that uses metal-coded mass tags (MMTs) to quantify\nthe delivery of protein into cells by several different PNC designs\nsimultaneously. Our approach will facilitate the development of more\npotent delivery systems by improving precision and reducing costs,\neffort, and time.