A Novel Label-Free Method for Efficient Detection\nof Bisphosphonate Drug Dissolution Rates Using Surface-Enhanced Raman\nSpectroscopy

We developed a novel method utilizing surface-enhanced\nRaman spectroscopy\n(SERS) with calcium ion-modified silver nanoparticles as the enhancing\nsubstrate. This approach enables label-free detection of the dissolution\nrate of bisphosphonates. This technique is fast, sensitive, and highly\nreproducible, significantly advancing drug quality control. Experimental\nresults demonstrated detection limits for etidronate, alendronate,\nand risedronate as low as 1 ng/mL. Notably, this method accurately\nidentifies characteristic drug peaks without interference from excipients,\nallowing for precise quantitative detection. In summary, our SERS-based\nmethod offers a simple, efficient, and reliable approach for the qualitative\nand quantitative quality control of bisphosphonate drugs.