Additional file 2 of Encrustations on ureteral stents from patients without urinary tract infection reveal distinct urotypes and a low bacterial load

Figure S1. Additional SEM images representing the presence of fungi, coverage by EPS and bacterial load. Figure S2. a Standard curve of serially diluted recDNA containing the E. coli 16S rRNA gene (data from 14 independent experiments). The 16S rRNA gene copy number was plotted against the calculated gene copy number (slope = 1.03011 ± 0.07591, intercept − 0.16974 ± 0.59028, R2 = 0.98397, n = 14). Inter-run calibration was performed using the recDNA. b Bacteria equivalents assessed by NGS plotted vs. 16S rRNA gene copies as assessed by qPCR. 16S qPCR correlated with normalized NGS reads (Spearman ranks r = 0.620, p < 0.0001, 95% CI 0.4642 to 0.7390). Table S3. OTUs present in more than 50% of the samples. Figure S4. Rarefaction curves of the 86 sequenced samples. Figure S5. XRD signals and comparison with expected patterns from the COD database. (DOCX 2772 kb)