Additional file 1 of Rare but specific: 5-bp composite motifs define SMAD binding in BMP signaling

Additional file 1: Supplementary Figs. S1–S7. Fig. S1 SMAD-MH1 purity control and experimental set-up. Fig. S2 BRE2-Luc and CAGA12-Luc define experimental conditions for selective comparison of BMP6 and TGFβ1. Fig. S3 BMP6 dose–response curves of SMAD composite motif reporters. Fig. S4 Composite motif spacers below 5 bp do not inhibit SMAD1-MH1 double binding. Fig. S5 BMP-responsive SMAD homocomposite motif reporters are unresponsive to TGFβ1. Fig. S6 BMP-responsive SMAD heterocomposite motif reporters are unresponsive to TGFβ1. Fig. S7 BMP specificity of SMAD composite motif reporters is maintained in U2OS cells.