Additional file 2 of ASFV pS183L protein negatively regulates RLR-mediated antiviral signalling by blocking MDA5 oligomerisation

Additional file 2. Both LMW poly(I:C) and HMW poly(I:C) up-regulate MDA5 and RIG-I transcription. PK15 cells were transfected with LMW poly(I:C) or HMW poly(I:C) (10 μg/mL) for the indicated times. The cells were then harvested for RNA extraction. Semiquantitative PCR was carried out to detect MDA5 and RIG-I.

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Publication Details

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Figshare
Published
2025-01-01
DOI
https://doi.org/10.6084/m9.figshare.28703483
Primary Topic
Cell Image Analysis Techniques
Type
article
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article

Additional file 2 of ASFV pS183L protein negatively regulates RLR-mediated antiviral signalling by blocking MDA5 oligomerisation

Figshare
Cell Image Analysis Techniques
article

Additional file 2 of ASFV pS183L protein negatively regulates RLR-mediated antiviral signalling by blocking MDA5 oligomerisation

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Abstract

Additional file 2. Both LMW poly(I:C) and HMW poly(I:C) up-regulate MDA5 and RIG-I transcription. PK15 cells were transfected with LMW poly(I:C) or HMW poly(I:C) (10 μg/mL) for the indicated times. The cells were then harvested for RNA extraction. Semiquantitative PCR was carried out to detect MDA5 and RIG-I.

Figshare
Nanjing Agricultural University (CN), University of Alberta (CA), China Pharmaceutical University (CN), Jiangsu Agri-animal Husbandry Vocational College (CN), China Institute of Veterinary Drug Control (CN)
Openalex Percentile: Top 54%
Cell Image Analysis Techniques
DOIOpen Access PDFOpenAlex
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