Additional file 1 of Initial gut microbiota composition is a determining factor in the promotion of colorectal cancer by oral iron supplementation: evidence from a murine model

Additional file 1. Supplementary Figure S1. Dietary iron supplementation promotes duodenal tumorigenesis in ApcMin/+mice independently of fecal microbiota transplant origin. Supplementary Figure S2: Representative pictures of the colon of ApcMin/+mice.Supplementary Figure S3: CRC associated gut microbiota differ from healthy control gut microbiota. Supplementary Figure S4: Bray-Curtis distances. Supplementary Figure S5: Beta diversity analysis of FMT-HC and FMT-CRC as a function of the dietary iron and initial gut microbiota composition. Supplementary Figure S6: Alpha-diversity indexes. Supplementary Figure S7: Quantification of B. pseudolongum and R. ilealis in the gut microbiota of FMT-CRC ApcMin/+mice. Supplementary Figure S8: Quantification of SCFA in the colon of FMT-CRC ApcMin/+mice supplemented withA. inops and B. pseudolongum. Supplementary Figure S9: Colonic cytokines levels in FMT-CRC mice. Supplementary Table S1: Demographic, clinical, and perioperative data of patients with colorectal cancer and healthy controls Supplementary Table S2: Erythroid parameters of patients with colorectal cancer. Supplementary Table S3: Primers for real-time PCR. Supplementary Table S4: Significant changes in relative abundances of microbial taxa at the family level.