Additional file 12 of γ-Secretase modulator resistance of an aggressive Alzheimer-causing presenilin mutant can be overcome in the heterozygous patient state by a set of advanced compounds

Supplementary Material 12: Figure S12. Levels of Aβ secreted by iPSC-derived neurons. (A-C) Measured amounts (pg/ml) of Aβ37 (A), Aβ38 (B) and Aβ40 (C) in the medium of WT (WT/WT), heterozygous (WT/L166P) or homozygous (L166P/L166P) PS1 L166P KI neurons that were treated with DMSO, RO7019009 (500 nM and 2.5 µM) or RO5254601 (2.5 µM) (n = 10–14). Aβ species were measured by species-specific Aβ ELISA (IBL) (Aβ38 and Aβ40) or electrochemiluminescence immunoassay (MSD, Aβ37) and are presented as mean + SEM. The dashed line highlights the levels of the DMSO vehicle-treated WT control. Missing data points are due to Aβ levels below the detection limit of the assay.