Additional file 5 of m5C methylation of mitochondrial RNA and non-coding RNA by NSUN3 is associated with variant gene expression and asexual blood-stage development in Plasmodium falciparum

Supplementary Material 5: Fig. S1 Sequence alignment of the catalytic domain of the eukaryotic NSUN family. Fig. S2 The PfNSUN3-Ty1-Ribo fusion gene triggered a knockdown effect at the protein level. (A) Western blot of total protein extracts from ring (R), trophozoite (T), and schizont (S) stages from the PfNSUN3-Ty1-Ribo strain with or without culture GlcN. (B–D) Global comparison of expression levels for all genes in the PfNSUN3-Ty1-Ribo line with or without drug at different IDC stages. (E) The transcriptome changes in the var gene family of the PfNSUN3-Ty1-Ribo line with or without drug at the ring stage. (F) Histogram displaying the number of up- or downregulated DEGs through the different IDC stages.