Additional file 1 of In vivo imaging of the spatial heterogeneity of intratumoral acidosis (pH) as a marker of the metastatic phenotype in breast cancer

Supplementary Material 1: Supplementary Fig. 1. Basal (A) and compensatory (B) glycolysis rates were extrapolated from the Glyco rate assay and normalized to the protein content. Data represent the means ± SEMs. n = 3 biological replicates in at least either duplicate or technical triplicate. Student’s t-test. Basal (C) and maximal (D) respiration rates were extrapolated from the Mito Stress Test and normalized to the protein content. Data represent the means ± SEMs. n = 3 biological replicates in at least either duplicate or technical triplicate. Student’s t-test. *** P < 0.001; **** P < 0.0001. Supplementary Fig. 2. 4T1 tumor-bearing mice developed a greater number of lung metastases. Representative H&E staining of lung metastases from 4T1 (top) and 67NR (bottom) tumor-bearing mice. Amplification x4. Supplementary Fig. 3. Correlation of the tumor acidity score with TGR.