Additional file 1 of Production of alkaline lipase by Aspergillus terreus AUMC 15762 for laundry application

Supplementary Material 1. Table S1: Screening of independent parameters and their levels used in PBD; Table S2: The influencing factors and their levels in Box–Behnken design BBD; Table S3: Preliminary and secondary screening of the lipolytic activity by 141 fungal isolates from 15 Desert soil samples collected from Sohag, Qena, and Aswan Governorates during February 2019; Table S4: ANOVA for selected factorial model; Table S5: ANOVA test results for lipase production; Table S6: Effects of metal ions on the activity of the pure lipase produced by A. terreus AUMC 15762 (Mean values (±SD) with different letters are significantly different (p ≤ 0.05; n = 3); Fig. S1: Fig. S1 Aspergillus terreus AUMC 15762. (A), Seven-day-old colonies on CYA at 25 °C (B), conidiophores and conidial heads. (C), smooth, globose conidia (Scale bars = 20 µm). Fig. S2: Purification of lipase in crude extract by anion exchange resin Flow rate = 15 ml/h, Fraction volume = 3 ml; Eluent = 100 mM phosphate buffer (pH 8.0); Fig. S3: Line weaver–Burk plot for the pure lipase produced by A. terreus AUMC 15762.