Additional file 1 of Palmitate enhances MSC immunomodulation of human macrophages via the ceramide/CCL2 axis in vitro

Supplementary Material 1: Supplementary Figure 1: Characterisation of MDM surface factors via flow cytometry. Human peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats, monocytes were selected via plastic adherence and differentiated into monocyte-derived macrophages (MDMs) over 6 days. MDMs were then detached using a lidocaine detachment buffer, stained for CD14, CD86, and CD206, and analysed by flow cytometry. Some MDMs were stimulated with 100 ng/mL LPS to observe changes in CD86 expression. n = 3 (3 different PBMC donors)