Additional file 1 of Metabolic engineering of Yarrowia lipolytica for the production and secretion of the saffron ingredient crocetin

Additional file 1: Figure S1. Overview of metabolic engineering strategies to improve crocetin production in microorganisms. Figure S2. Strain construction tree in this research. Figure S3. Standard curves for carotenoid quantification by HPLC. Figure S4. Absorbance spectra of supernatants of β-carotene and crocetin-producing strains after fermentation at 30°C for 7 days. Figure S5. Extracellular and intracellular analysis of carotenoids. Figure S6. Culture broth of crocetin producer under different conditions. Figure S7. Analysis of cell death during crocetin fermentation using YLC01 strain. Figure S8. Introducing an exogenous ALD. Figure. S9 Effects of glucose concentration on crocetin contents in Y. lipolytica YLC01. Table S1. Biosynthetic genes used in this research. Table S2. Primers used in this research. Table S3. All yeast strains and plasmids constructed in this research. Table S4. Media optimization based on YPD and calculation of C/N ratios.