Additional file 1 of Chlorella vulgaris mutants with altered cell walls show increased permeability and enhanced extractability of intracellular molecules

Supplementary material 1. Figure S1. Mechanism of action of fluorescent probes used and schematic representation of the screening process. Figure S2. Dependence of the retention of fluorescent probe on cell vitality and cell wall integrity. Figure S3. Setup of a screening strategy for isolating C. vulgaris mutants with increased cell wall permeability (CWP), utilizing flow cytometry. Figure S4. Example of sorting of EMS-mutagenized cell populations based on FDA and EB fluorescence. Figure S5. Summary table of parameters for selecting the 6 putative CWP mutants. Figure S6. FTIR spectroscopy of cell wall components. Figure S7. Fluorescence emission of CWP1 cells stained with Calcofluor White. Figure S8. Sorting of EMS-mutagenized CWP1 cells based on Cf fluorescence. Figure S9. Setup of a bead-beating method for protein extraction from Cv biomass. Figure S10. Characterization of mechanical resistance in Cv lines selected for lower Cf binding affinity. Figure S11. Permeability of CWP1 and CFW cells to fluorescent probes. Table S1. Data output from sorting performed by the FACSAria Fusion, for isolating CWP mutants.