Additional file 2 of Identification of the methionine transporter MetQ in Streptococcus suis and its contribution to virulence and biofilm formation

Additional file 2. Alignment of MetQ proteins across various bacterial species. The alignment includes MetQ from S. suis strain P1/7 (SSU1577), S. pneumoniae strain 284 (ACGQOV_002047), Neisseria meningitidis strain BZ133 (GNA1946), Escherichia coli strain NCTC9081 (NCTC9081_02188), and Treponema pallidum strain TPANIC_082. Residues highlighted in red indicate amino acids involved in substrate binding as identified in T. pallidum. A residue highlighted in green indicates an essential substrate binding site identified in E. coli and N. meningitidis. Residues involved in protein substrate interaction, as identified in N. meningitidis, are marked in red. An asterisk indicates a residue conserved across all strains, a single dot marks residues shared by at least two strains, and two dots mark residues shared by three or more strains. Green arrows indicate the start and end points of the sequence of the recombinant MetQ, which is fused in its N-terminal extreme to MCGGHHHHHHHH, as encoded by the plasmid.