Additional file 1 of Gut microbiota-mediated bile acid transformations regulate the transport of aflatoxin B1 from the intestine to the liver in piglets

Additional file 1: Method S1. 16S rRNA amplicon sequencing and data analyses. Method S2. Bile acid analysis. Method S3. RNA-seq. Table S1. Primer sequences used for Cyp8b1-KO genotyping. Table S2. Average recovery rates of AFB1 in samples. Table S3. Primer sequences for qPCR analysis. Table S4. Dissimilarities of colonic microbiota between piglets in different groups revealed by ANOSIM based on Bray-Curtis distance. Table S5. Dissimilarities of ileal microbiota between piglets in different groups revealed by ANOSIM based on Bray-Curtis distance. Fig. S1. ABX cleared the gut microbiota of piglets on d 3. Fig. S2. Effect of AFB1 and ABX treatments on piglets. Fig. S3. Effect of AFB1 and ABX on the intestinal barrier of piglets. Fig. S4. Overview of RNA-seq. Fig. S5. Effect of AFB1 and ABX treatment on colonic microbiota of piglets. Fig. S6. Effect of AFB1 and ABX treatment on ileal microbiota of piglets. Fig. S7. Effect of AFB1 and OCA treatments on piglets. Fig. S8. Results of Cyp8b1-KO mice genotyping. Fig. S9. Effect of AFB1 treatment on WT mice and Cyp8b1-KO mice.