Additional file 7 of Glutathione reductase modulates endogenous oxidative stress and affects growth and virulence in Avibacterium paragallinarum

Additional file 7. LC–MS/MS metabolomics analysis unveils alterations in metabolic functions and pathways in Av. paragallinarum associated with GR knockout. A OPLS-DA plot showing significant metabolic differences between WT and ΔGR strains. B Heatmap showing the cluster analysis of metabolite levels in WT and ΔGR strains. C Heatmap analysis of the 14 identified upregulated metabolites. D Chord diagram showing the metabolic pathways and the interconnectivity of the significantly altered metabolites in the ΔGR strain. E Network of differential metabolites displaying the coordinated metabolic response to GR knockout. F KEGG classification analysis of differentially regulated metabolites between WT and ΔGR strains of Av. paragallinarum.