Additional file 4 of Comparing Xenium 5K and Visium HD data from identical tissue slide at a pathological perspective

Supplementary Material 4: Supplementary Fig. 4. Comparison of Visium-HD and Xenium-5K performance in analyzing irregular structures and pigmented cells. (a) Side-by-side H&E, Xenium-5K, Visium-HD, and single-cell–re-segmented Visium-HD views of the same normal-lung field, with color-coded clusters. (b) Overlay of H&E and Xenium-5K data highlighting AT1 and AT2 cells. Heatmap shows differentially expressed genes between clusters 18 (AT1) and 20 (AT2). (c) Overlay of H&E and Xenium-5K with high endothelial venule (HEV) colored in green. Visium-HD of the same region is shown in the right with colored individual clusters. (d) Spatial transcript-density maps of a dust-cell–rich (pigmented macrophage) area rendered with Visium-HD and Xenium-5K.