Antioxidant and Anti-Aging Effects of <i>Aquilaria crassna</i> Heartwood Ethanol Extract in <i>Saccharomyces cerevisiae</i>

<i>Aquilaria crassna</i>, a medicinal plant, has long been used in traditional medicine for its remarkable health benefits. This study investigated the anti-aging effects of <i>Aquilaria crassna</i> Heartwood Ethanol Extract (ACHEE) in yeast <i>Saccharomyces cerevisiae</i>. ACHEE improved yeast’s chronological lifespan by reducing levels of reactive oxygen species, improving mitochondrial membrane potential, and decreasing apoptosis. The mRNA expression levels of <i>TOR1</i> and <i>SCH9</i> genes were lowered, while those of <i>RIM15</i> and <i>SIR2</i> genes were increased upon treatment with extract. Together with molecular docking analysis, the findings suggest ACHEE exerts anti-aging effects via modulating antioxidant and nutrient sensing pathways.