Additional file 7 of Cytotoxicity induced by Aeromonas schubertii is orchestrated by a unique set of type III secretion system effectors

Additional file 7. Western blot analysis of caspase-3 activation. HeLa cells were either left uninfected or infected with A. schubertii wild-type (WT) or mutant strains lacking the API1 (ΔAPI1) or API2 (ΔAPI2) injectisomes, at an MOI of 10:1. One hour post-infection, the extracellular bacteria were eliminated by the addition of gentamicin. Whole-cell lysates were prepared at indicated time points, separated by SDS-PAGE, and analyzed by immunoblotting using an antibody that detects both the full-length inactive form of caspase-3 (pro-casp 3, 35 kDa) and the cleaved caspase-3 fragment (cleaved casp 3, 17 kDa). βactin (40 kDa) was used as a loading control. HeLa cells treated with 1 µM staurosporine (STS) served as a positive control. Data are representative of 2 independent experiments.