Additional file 2 of Unflavored electronic cigarette exposure induces alterations in airway ciliary structure and function

Supplementary Material 2: Supplemental Figure 2 is associated with Figure 3. Figure 2S: Ciliary beat frequency, differential gene expression and quality control analyses of phosphoproteomics data after exposure to unflavored e-cig aerosols. (A) Percentage ciliary movement in ALI cultures from donor 1 (cyan) and donor 2 (magenta) after unflavored e-cig exposure or room air control. (B) Ciliary beat frequency in ALI cultures from donor 1 (cyan) and donor 2 (magenta) after unflavored e-cig exposure or room air control. (C) List of differential gene expression after unflavored e-cig exposure as compared to room air control. (n=2 biological replicates). (D) Gene ontology terms related to molecular function after unflavored e-cig exposure as compared to room air control. (E) Table of proteins that were changed in abundance by exposure to unflavored e-cigs. (n=2 biological replicates). (F) Principal component analysis (PCA) for each replicate of air and unflavored e-cig conditions. (G) Number of total detected phosphorylated and non-phosphorylated peptides from the phosphoproteomics analysis in each replicate for air and unflavored e-cig conditions. (G) graph showing phosphopeptide enrichment efficiency in each sample. (n=1 biological replicate, 3 technical replicates)