Additional file 5 of African swine fever virus MGF505-4R facilitates cGAS degradation through TOLLIP-mediated selective autophagy and inhibits the formation of ISGF3 to evade innate immunity

Additional file 5. Validation of siRNA knockdown effects. (A) HeLa cells were transfected with either negative control siRNA (siNC) or siRNA targeting cGAS (sicGAS) for 24 h before western blot analysis. (B) HeLa cells were transfected with siNC or siRNA targeting ATG5 (siATG5) for 24 h before western blot analysis. (C) HeLa cells were transfected with siNC or siRNA targeting TOLLIP (siTOLLIP) for 24 h before western blot analysis.