Additional file 2 of Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors

Supplementary file 8. Figure S5 Protein model based on DmelIR64a generated by RasTop and magnified view of the ligand-binding domain. Lilac ribbons: S1-LBD; light-blue ribbons: S2-LBD. Red residues: positions of the conserved Arg402 [12, 42], and of the Lys623 residuethat in Supplementary Figure S4 we have indicated to be conserved among most of the acid sensing IRs. Yellow residues: amino acid substitutions within the S1/S2 between the D. melanogaster and D. suzukii orthologues, as indicated in Figure 5: Val/Leu312 and Leu/Ser414 in the S1, Ser/Thr632, Pro/Ser689, Ala/Ser710 and Thr/Ser791 in the S2. Note: the PDB accession Q9 VRL4 provides a protein, which amino acid at position 414 is a Serine as in its D. suzukii orthologue. Note: except for Arg402, all of the indicated residues are distant from the S1/S2 pocket of the LBD.