Additional file 2 of Dietary L-leucine supplementation improves ruminal fermentation parameters and epithelium development in fattening Angus beef cattle

Additional file 2: Fig. S1. The α-diversity indices including observed genus, A and B Chao and Shannon indexes (n = 6); C Principal coordinates analysis (PCoA). D Non-metric multidimensional scaling (NMDS). Fig. S2. Results of metagenomic sequencing of the rumen bacteria in the CON and Leu (n = 6). A Differences in bacterial phylum levels. B Differences in bacterial species levels.*P < 0.05, **P< 0.01, ***P < 0.001. Fig. S3. Classification of rumen differential metabolites by KEGG compound database (CON/Leu, variable importance in the projection [VIP] > 1.5, P < 0.05) (n = 5).