Additional file 16 of The double homeodomain protein DUX4c is associated with regenerating muscle fibers and RNA-binding proteins

Additional file 16: Figure S15. Partial co-localization of DUX4c with RNA binding proteins in differentiating muscle cells. Human testis sections were used. (A) Immunohistochemistry was performed as in Fig. S5B. The small boxed areas on the images are shown below at higher magnification. (Sg: Spermatogonia; Ser: Sertoli cells; SpI: spermatocytes I; St(1): early spermatids; St(2) late spermatids; Sz: spermatozoa). Arrows and arrowheads indicate DUX4c localization. (B-D) Immunofluorescence was performed as in Figs. 3: DUX4c (green) and ILF3/NF90 (red) were detected with specific primary antisera (see Methods) and appropriate secondary antibodies coupled to Alexa Fluor 488 or 555 respectively. (B) The boxed regions correspond to DUX4c/ILF3 partial co-localization. The star indicates a large spermatocyte I nucleus with DUX4c- and ILF3-positive nuclear spots. (C) Arrows indicate DUX4c/ILF3 partial co-localization at the nuclear periphery of a round spermatid (higher magnification of the boxed region in C in another Z axis image.) (D) Elongating spermatids (arrowheads) showing ILF3 cytoplasmic labeling. The arrow points to diffuse and weak cytoplasmic DUX4c staining.