Additional file 1 of Resourceful and economical designing of fermentation medium for lab and commercial strains of yeast from alternative feedstock: ‘transgenic oilcane’

Supplementary material 1: Table 1: Concentrations of minor chemicals present in growth media constructed using transgenic oilcane hydrolysate and juice blends prior to and post-fermentation. Table 2: a) Phospholipids in oilcane hydrolysate and juice obtained using untargeted LC–MS metabolite analysis. The results are reported as signal's peak area counts normalized to the internal standard's peak area count and/or sample volume/sample weight. b) LC/MS analysis of lipid classes, lipid ions and ion formulae of ten major lipids in hydrolysates and juice derived from transgenic oilcane. Table 3: Growth kinetics of xylose metabolizing S. cerevisiae strains in aerobic cultures without acclimated strains. The medium was an equal blend of hydrolysate and juice. Fig. S1: Sugar consumption profile of metabolically engineered S. cerevisiae strains CT2-Pro, NKSW7-1and commercial yeaston 100% hydrolysateand 50:50 Hydrolysate: Juicederived from transgenic oilcane, Fig. S2: Sugar consumption profile of metabolically engineered S. cerevisiae strains CT2-Pro, NKSW7-1and commercial yeast. The strains were acclimated on 50:50 Hydrolysate: Juice media prior to fermentation. Hydrolysate: Juice 50:50 media was diluted to get a total sugar concentration of ~ 120 g/land ~ 75 g/l. Fig. S 3: Growth and fermentation profile of metabolically engineered S. cerevisiae strains CT2-Pro, NKSW7-1and commercial yeast. The strains were not acclimated prior to fermentation. Hydrolysate: Juice 50:50 media was diluted to get a total sugar concentration of ~ 120 g/land ~ 75 g/l.