Supplemental Table S3

Bulk mRNA-seq was performed using RNA isolated from ventricles of cryoinjured and sham-operated fish at 7 days post-cryoinjury (dpci) of wt and ankrd1a mutant fish. Three ventricles were pooled as one sample, and each group contained 4 samples. Differential expression analysis of two conditions was performed using a multi-factorial statistical analysis based on a negative binomial model that used a generalized linear model approach computed by edgeR (v4.2.0) from raw counts in each comparison (16 samples in 4 different groups). The bioMart package (v2.60.1) was used to annotate differentially expressed genes (DEGs). The RNA-seq data are deposited in NCBI´s Gene Expression Omnibus and are accessible through GEO Series accession number GSE285387 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE285387 ).