Additional file 6 of Pharmacological CLK inhibition disrupts SR protein function and RNA splicing blocking cell growth and migration in TNBC

Supplementary Material 6: Fig. 6. Identification of the SRSF7 interactome in MDA-MB-231 SRSF7-GFP expressing cells via GFP pulldown/MS. (A) The SRSF7 interactome in cells treated with 0.1% DMSO and (B) 1 µM T-025. Red points represent proteins enriched in the SRSF7 interactome with log2FC >1.0 and an FDR of < 0.05 as compared to MDA-MB-231-GFP cells. (C) The log2 fold change in the abundance of PRPFs, CLKs and RNA polymerase II subunits between cells treated with 1 µM T-025 and 0.1%DMSO. (D) Quantification of the input in GFP pulldown/Western blot