Additional file 3 of MMP28 recruits M2-type tumor-associated macrophages through MAPK/JNK signaling pathway-dependent cytokine secretion to promote the malignant progression of pancreatic cancer

Supplementary Material 3. Figure S3. MMP28 overexpression promotes the migration of TAMs and polarization of the M2 TAMs. (A) The ability of CM from cancer cells in the Vector group and the OE-MMP28 group to attract TAMs was assessed in 8 μm Transwell chambers. Compared with CM from the OE-MMP28 group, the effects of the addition of the JNK inhibitor SP600125 on the ability of cancer cells to promote TAM migration were determined. Scale bar, 100 μm. (B) qRT-PCR was used to determine the expression levels of CD86 and CD163 TAM markers, in different coculture groups. (C) The expression levels of the TAM markers CD86 and CD163 in different coculture groups were determined by immunofluorescence staining. Scale bar, 50 μm. (D) The proportions of CD11b+CD86+ TAMs and CD11b+CD163+ TAMs in different coculture groups were analysed by flow cytometry. *P<0.05, **P<0.01,***P<0.001, and ****P<0.0001. #P<0.05, ##P<0.01, and ####P<0.0001.