Additional file 1 of Unflavored electronic cigarette exposure induces alterations in airway ciliary structure and function

Supplementary Material 1: Supplemental Figure 1 is associated with Figure 1. Figure 1S: Flavor-to-flavor heterogeneity and donor-to-donor heterogeneity in response to flavors is evident in ALI cultures. (A) Quantification of number of Keratin 5+ in each donor separately across the six exposures. (B) Quantification of number of Ac-Tub+ in each donor separately across the six exposures. (C) Quantification of number of Muc5AC+ mucus cells in each donor separately across the six exposures. (D) Quantification of number of Keratin 5+ in response to each flavor across the three donors. (E) Quantification of number of Ac-Tub+ in response to each flavor across the three donors. (F) Quantification of number of Muc5AC+ mucus cells in response to each flavor across the three donors. Graphs represent mean ± SEM, n = 3 biological replicates (3 different donors), each with 3 technical replicates (3 ALI transwell cultures derived from each of the 3 different donors). Each dot represents a replicate. P values are calculated from all technical replicates across the biological replicates.