Additional file 6 of Effects of hypoxia stress on the milk synthesis in bovine mammary epithelial cells

Additional file 6: Fig. S6. Effects of HIF-1α knockdown on mTOR signaling proteins phosphorylation in BMECs. (A) Immunoblots of the phosphorylated forms (P) and total levels (T) of the mTOR signaling proteins in BMECs under 24 h hypoxia and transfected with either a control (NC) siRNA or HIF-1α siRNA (n = 3). (B–D) Quantitative analyses of the blot densities of the P/T forms of the mTOR signaling proteins in A. Data with error bars represent mean ± SEM. Statistical significance (P < 0.05) was determined by a two-tailed unpaired t-test.