Additional file 1 of Detoxification of coumarins by rumen anaerobic fungi: insights into microbial degradation pathways and agricultural applications

Supplementary Material 1: Fig. S1. Comparisons of gas production and metabolite concentrations from in vitro incubation of enriched rumen fungi and whole rumen microbiota with Melilotus officinalis as the substrate. Fig. S2. Dynamic changes of nutrients during Melilotus officinalis silage fermentation for 60 d. Fig. S3. Dynamic changes of nutrient retention rate and coumarin degradation rate of Melilotus officinalis silage fermented for 60 d. Fig. S4. Dynamic changes of pH value, lactateand NH3-Nconcentrations in Melilotus officinalis silage fermented for 60 d. Fig. S5. Effects of coumarinand glucoseon the biofilm morphology and metabolites of P. ruminantium F1. Fig. S6. Effects of coumarinon the transcripts and metabolic pathways of P. ruminantium F1. Fig. S7. Pathway enrichment analysis of genes from P. ruminantium F1 caused by the addition of 3 mmol/L coumarin at mid-exponential phase of fungal growth.