<b>First Mitochondrial Genome Draft of </b><b><i>Drosophila melanogaster</i></b><b> from Pakistan</b>

<i>Drosophila melanogaster</i> is one of the most employed animal models and amongst the first of the organisms selected for genome sequencing. Geographically isolated population of <i>D. melanogaster</i> have shown to harbour distinct allelic makeup. Herein, we present the first whole mitochondrial genome sequence draft of a white eyed mutant <i>D. melanogaster</i> dubbed as ANU-1, isolated from wildtype population from Karachi-Pakistan. The complete mitochondrial genome of <i>D. melanogaster</i> ANU-1 is 19,116 bp in length (accession number OR555819.1) containing 13 protein coding genes, 22 tRNA genes, 2 rRNA genes and an AT-rich region of 4208 bp in length. Phylogenetic analysis clustered the mitochondrial sequence of <i>D. melanogaster</i> ANU-1 with melanogaster subgroup. Genome sequence comparison identified novel alleles in<i> COX1, ND5</i> and <i>CYTB</i> genes in <i>D. melanogaster</i> ANU-1 with subtle structural variations. The presented mitochondrial genome provides the first glimpse of allelic variations of <i>D. melanogaster</i> from Pakistan.