Additional file 6 of C9ORF72 poly-PR disrupts expression of ALS/FTD-implicated STMN2 through SRSF7

Supplementary file 6: Figure S3: Effect of DPRs on SRSF7 protein level and selected transcripts encoding RNA binding proteins. A) Immunoblot of SRSF7 protein from cells transfected with DPRs: GFP-(GA)50, GFP-(GR)50, GFP-(PR)50 or GFP alone (control). B) Quantification of SRSF7 levels normalized to GAPDH from three independent transfections (two-tailed t-test, ns: not significant). C) Quantitative PCR for selected transcripts encoding RNA binding proteins normalized to housekeeping genes (GAPDH, ACTB) in cells transfected with DPRs or GFP alone (control) from three independent transfections. (Two-way ANOVA with Dunnett’s multiple comparisons test; * adj. p < 0.05, ** adj. p < 0.01, *** adj. p < 0.001, else non-significant).