Additional file 1 of Co-expression of auxiliary genes enhances the activity of a heterologous O2-tolerant hydrogenase in the cyanobacterium Synechocystis sp. PCC 6803

Supplementary Material 1: Fig. S1. Overview of the cloning strategy. Fig. S2. PCR to confirm the correct generation of Syn_PnrsBReSHp strain. Fig. S3. SDS-PAGE loading control for Western blot analysis of expression levels with different inducer concentrations. Fig. S4. SDS-PAGE as loading control for Western Blot analysis of expression levels at different time points after induction. Fig. S5. Soluble cell-free extract from Syn_∆hox used as negative control. Fig. S6. (RT)-PCR targeting hypX. Fig. S7. Growth curves of not induced and induced strains expressing C. necator hydrogenase and maturases. Fig. S8. Characterization of Syn_PnsrBCnSHg containing pPnrsBCnHypX or pPrhaBADCnHypX. Table S1. Protocol for plasmid generation and propagation in E. coli DH5α. Table S2. Sequences of primers used in this study and of basal genetic elements implemented in our MoClo system. Table S3. List of plasmids generated in this work. Table S4. Strains used in this study. Table S5. Gas concentrations during in vivo H2 consumption assay. Table S6. H2 uptake rate during H2 consumption assay.