Additional file 1 of Viable bacterial communities in freshly pumped human milk and their changes during cold storage conditions

Additional file 1: Figure S1. Sequencing depth with alpha rarefaction curves in all analyzed samples (n = 142). Samples with PMA treatment (+ PMA; only viable genera) as well as without PMA treatment (-PMA; both viable and non-viable bacterial genera) are shown. Figure S2. Descriptive statistics for sequencing depth, Shannon diversity index, and centered log-ratio (CLR)–transformed relative abundances of selected genera, stratified by sample matrix, time point, and PMA treatment group. All values are rounded to two decimals. Figure S3. Triangle heatmap of viable and non-viable bacterial genera in human milk samples. This heatmap displays the genus-level microbial composition of fresh human milk samples and milk samples stored under different conditions, i.e., at 4 °C for 24 and 48 h, and at -20 °C for six weeks. The relative abundances have been transformed using a centered log-ratio (CLR) approach. The top annotation provides a paired comparison of the Shannon index (calculated after rarefaction to 10,000 reads) and the sequencing depth (displayed on a log₁₀ scale, note that the blue and red bars overlap). In the split cells, the top-left triangle represents samples without PMA treatment (-PMA), while the bottom-right triangle represents PMA-treated samples (+ PMA).